slrA

slrA
168

negative regulator of the fla-che operon, anti-repressor, antagonist SinR for the repression of the epsA→epsO and tapA-sipW-tasA operons

Locus
BSU_38229
Molecular weight
6.00 kDa
Isoelectric point
9
Protein length
52 aaSequenceBlast
Gene length
159 bpSequenceBlast
Function
control of motility and biofilm formation
Product
antagonist of SinR and SlrR
Essential
no
Synonyms
slrA
Outlinks
BsubCyc UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
3,923,319 → 3,923,477
RNA
Additional information
the mRNA has long 5' and 3' UTRs (170 and 330 nt, respectively) PubMed
The protein
Catalyzed reaction/ biological activity
binding of SinR resulting in induction of SinR-repressed genes and operons PubMed
Domains
Sin domain (aa 1-38) (according to UniProt)
Structure
P0C8M5 (AlphaFold)
Effectors of protein activity
SlrR inhibits activity of SlrA (i. e. binding to SinR) PubMed
Paralogous protein(s)
SinI
Expression and Regulation
Operons
Genes
slrA
Description
PubMed
Regulatory mechanism
YwcC: repression, PubMed, in ywcC regulon
Additional information
increased expression in a pnpA mutant PubMed
half-life of the mRNA: 0.56 min, this increases to 1.06 in a pnpA mutant PubMed
RNA degradation by PnpA is proceeds from the 3' end of the mRNA through the Rho-dependent terminator PubMed
transcription termination depends on Rho PubMed
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slrA

2025-03-17 15:06:01

Jstuelk

78

D065C538DD2374540994AAB68777DD0B8BAE3B26

B6AFE071BE7A0BC5E2E9A62AB13D29C644E1D21B

Biological materials
Mutant
BKE38229 (ΔslrA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTAACCTCCAATTGTA,  downstream forward: _UP4_TAGTCCGAACAGGCGGATCT
BKK38229 (ΔslrA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTAACCTCCAATTGTA,  downstream forward: _UP4_TAGTCCGAACAGGCGGATCT
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
References
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C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7

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Time of last update: 2025-04-06 02:03:55

Author of last update: Jstuelk