sacP

sacP
168

sucrose permease of the phosphotransferase system, EIIBC of the PTS, Trigger enzyme, control of SacT activity

Locus
BSU_38050
Molecular weight
49.29 kDa
Isoelectric point
7.03
Protein length
461 aaSequenceBlast
Gene length
1386 bpSequenceBlast
Function
sucrose uptake and phosphorylation, control of SacT activity
Product
sucrose-specific PTS permease, EIIBC component
Essential
no
E.C.
2.7.1.69
Synonyms
sacP, ipa-49d
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,903,646  3,905,031
The protein
Protein family
PTS permease, sucrose family PubMed
Domains
PTS EIIB domain type-1 (aa 1-87) (according to UniProt)
PTS EIIC domain type-1 (aa 107-461) (according to UniProt)
Structure
P05306 (AlphaFold)
Paralogous protein(s)
TreP, SacX, BglP, MurP
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
sacP-sacA-ywdA
Description
PubMed
Regulation
induced by sucrose (SacT) PubMed
Regulatory mechanism
SacT: antitermination, via binding to a RNA switch, in sacT regulon
CcpA: repression, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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sacP→ywdA

2025-03-27 14:07:48

ghost

94

84ac6df24eedff839607263961d011c324b7f03e

98AC56561C7DAA3F5751178FDFB48FF02310E968

Biological materials
Mutant
BKE38050 (sacP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTTCTCCCCCTTTT,  downstream forward: _UP4_AATGAGGATGAGGAGAGGAA
BKK38050 (sacP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTTCTCCCCCTTTT,  downstream forward: _UP4_AATGAGGATGAGGAGAGGAA
Expression vectors
for expression, purification of the EIIB domain in E. coli with N-terminal His-tag, in pWH844: pGP429, available in Jörg Stülke's lab
for expression, purification of the EIIB domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP436, available in Jörg Stülke's lab
References
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AE02C38397AA790E4B216BB6DBABFE907B984D05

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Time of last update: 2025-04-06 13:21:48

Author of last update: Melvin.boenninger