narG

narG
168

nitrate reductase (alpha subunit)

Locus
BSU_37280
Molecular weight
138.86 kDa
Isoelectric point
6.06
Protein length
1228 aaSequenceBlast
Gene length
3687 bpSequenceBlast
Function
nitrate respiration, nitrogen assimilation
Product
nitrate reductase (alpha subunit)
Essential
no
Synonyms
narG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5013 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,826,259 → 3,829,945
The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
Protein family
prokaryotic molybdopterin-containing oxidoreductase family (according to UniProt)
Domains
4Fe-4S Mo/W bis-MGD-type domain (aa 47-111) (according to UniProt)
Cofactors
Fe-S cluster PubMed
MoCo PubMed
Structure
1Q16 (PDB) (the NarG-NarH-NarI complex of E. coli, 52% identity) PubMed
P42175 (AlphaFold)
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
narG-narH-narJ-narI
Description
PubMed
Regulation
expressed under anaerobic conditions (Fnr) PubMed
Regulatory mechanism
Fnr: activation, PubMed, in fnr regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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narG→narI

2025-04-04 05:20:34

ghost

94

2df0c9c15bd640f1035afac39ab71391c14fde4b

655A38484A21CE64690D3913F055D0C46476DC9F

Biological materials
Mutant
BKE37280 (ΔnarG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC,  downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
BKK37280 (ΔnarG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC,  downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
References
Reviews
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Original Publications
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B992698A12EE9D17DCEF075F82396D5456D35A20

Page visits: 5865

Time of last update: 2025-04-11 04:33:35

Author of last update: Jstuelk