narH

narH
168

nitrate reductase (beta subunit)

Locus
BSU_37270
Molecular weight
55.30 kDa
Isoelectric point
5.11
Protein length
487 aaSequenceBlast
Gene length
1464 bpSequenceBlast
Function
nitrate respiration, nitrogen assimilation
Product
nitrate reductase (beta subunit)
Essential
no
Synonyms
narH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1140 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,824,806  3,826,269
The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
Domains
3 4Fe-4S ferredoxin-type domains (aa 7-36, aa 172-203, aa 205-234) (according to UniProt)
Cofactors
Fe-S cluster PubMed
Structure
1Q16 (PDB) (the NarG-NarH-NarI complex of E. coli, 59% identity) PubMed
P42176 (AlphaFold)
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
narG-narH-narJ-narI
Description
PubMed
Regulation
expressed under anaerobic conditions (Fnr) PubMed
Regulatory mechanism
Fnr: activation, PubMed, in fnr regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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narG→narI

2025-04-04 05:20:34

ghost

94

2df0c9c15bd640f1035afac39ab71391c14fde4b

655A38484A21CE64690D3913F055D0C46476DC9F

Biological materials
Mutant
BKE37270 (narH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCATGACCATACCGATTT,  downstream forward: _UP4_TAACTTTTGATAAATGAGGT
BKK37270 (narH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCATGACCATACCGATTT,  downstream forward: _UP4_TAACTTTTGATAAATGAGGT
References
Reviews
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Original Publications
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6181C785427277C097B81D67A93213AEAF701FF4

Page visits: 3569

Time of last update: 2025-04-11 04:16:57

Author of last update: Jstuelk