cssS

cssS
168

two-component sensor kinase, control of cellular responses to protein secretion stress

Locus
BSU_33020
Molecular weight
51.92 kDa
Isoelectric point
5.8
Protein length
451 aaSequenceBlast
Gene length
1356 bpSequenceBlast
Function
control of cellular responses to protein secretion stress
Product
two-component sensor kinase
Essential
no
Synonyms
cssS, yvqB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,386,398  3,387,753
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of CssR
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
Domains
two transmembrane segments, C-terminal histidine phosphotransferase domain
HAMP domain (aa 287-239) (according to UniProt)
Histidine kinase domain (aa 247-451) (according to UniProt)
Structure
O32193 (AlphaFold)
Modification
autophosphorylation on a His residue
Effectors of protein activity
the extracellular loop domain is required for signal perception PubMed
Paralogous protein(s)
YclK, YvrG, YkoH, KinE
Localization
cell membrane (according to Swiss-Prot)
localized primarily at the division septum but also found in a punctate pattern with lower intensity throughout the cell cylinder  PubMed
Expression and Regulation
Operons
Genes
cssR-cssS
Description
PubMed
Regulation
expressed under conditions of secretion stress (CssR) PubMed
Regulatory mechanism
CssR: activation, PubMed, in cssR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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cssR→cssS

2025-04-04 08:16:04

ghost

96

d62586dd7176a72b7cf49ae683ed93d09bb4b382

2C457B4F2C19BB95984674749FFD2EB045C6C14F

Biological materials
Mutant
MGNA-B216 (yvqB::erm), available at the NBRP B. subtilis, Japan
BKE33020 (cssS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATGATGACATCATCCT,  downstream forward: _UP4_TAGACTGTAGATGTTTTGCA
BKK33020 (cssS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATGATGACATCATCCT,  downstream forward: _UP4_TAGACTGTAGATGTTTTGCA
References
Reviews
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Original Publications
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4EE48E4931F662E51586DB6D01E91C688329222D

Page visits: 4244

Time of last update: 2025-04-10 05:12:59

Author of last update: Jstuelk