Difference between revisions of "AmyX"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29930&redirect=T BSU29930] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/amyX.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/amyX.html] | ||
| Line 95: | Line 96: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU29930&redirect=T BSU29930] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2E8Z 2E8Z] (complex with alpha cyclodextrin), [http://www.rcsb.org/pdb/explore.do?structureId=2E9B 2E9B] (complex with maltose) | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2E8Z 2E8Z] (complex with alpha cyclodextrin), [http://www.rcsb.org/pdb/explore.do?structureId=2E9B 2E9B] (complex with maltose) | ||
Revision as of 14:32, 2 April 2014
- Description: pullulanase (debranching enzyme)
| Gene name | amyX |
| Synonyms | |
| Essential | no |
| Product | pullulanase |
| Function | starch degradation |
| Gene expression levels in SubtiExpress: amyX | |
| MW, pI | 80 kDa, 5.799 |
| Gene length, protein length | 2154 bp, 718 aa |
| Immediate neighbours | ytmP, ytlR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29930
Phenotypes of a mutant
Database entries
- BsubCyc: BSU29930
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): GlgB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU29930
- UniProt: C0SPA0
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jae-Hoon Shim, Jong-Tae Park, Jung-Sun Hong, Ki Woo Kim, Myo-Jeong Kim, Jung-Hyuk Auh, Young-Wan Kim, Cheon-Seok Park, Winfried Boos, Jung-Wan Kim, Kwan-Hwa Park
Role of maltogenic amylase and pullulanase in maltodextrin and glycogen metabolism of Bacillus subtilis 168.
J Bacteriol: 2009, 191(15);4835-44
[PubMed:19465663]
[WorldCat.org]
[DOI]
(I p)
Dominggus Malle, Takafumi Itoh, Wataru Hashimoto, Kousaku Murata, Shigeru Utsumi, Bunzo Mikami
Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 4);381-4
[PubMed:16582490]
[WorldCat.org]
[DOI]
(I p)
Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221]
[WorldCat.org]
[DOI]
(P p)
