ribR

ribR
168

riboflavin kinase with preference for dihydroriboflavin, binds FMN riboswitches of the rib operon and of ribU to allow expression even in the presence of FMN, required for the conversion of S-methyl-cysteine to cysteine

Locus
BSU_29300
Molecular weight
26.08 kDa
Isoelectric point
9.28
Protein length
230 aaSequenceBlast
Gene length
693 bpSequenceBlast
Function
utilization of S-methyl-cysteine, regulation of rib operon expression
Product
riboflavin kinase with preference for dihydroriboflavin
Essential
no
Synonyms
ribR, ytnK, rbfK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0196 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,000,985  3,001,677
Phenotypes of a mutant
no growth with S-methyl cysteine PubMed
The protein
Catalyzed reaction/ biological activity
synthesis of FMNH2 PubMed
binds the FMN riboswitch PubMed
prevents transcription termination of the ''ribD-ribE-ribA-ribH-ribT'' operon even in the presence of FMN or FMNH2 PubMed
prevents sequestration of the ribosome binding site of the ''ribU'' mRNA even in the presence of FMN or FMNH2 PubMed
ATP + riboflavin --> ADP + FMN + H+ (according to UniProt)
Protein family
RibR family (single member, according to UniProt)
Structure
P94465 (AlphaFold)
Paralogous protein(s)
RibC, (42%)
Expression and Regulation
Operons
Genes
snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
Description
PubMed
Regulation
induced in the presence of methionine and taurine PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
AscR: activation, PubMed, in ascR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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snaA→ytnM

2025-03-22 23:22:48

Jstuelk

115

8a3868e633778d2f68bf47d615f22f716e843294

4D6CA410A1DC104A491D3C18248CCD50CC0E983A

Biological materials
Mutant
MGNA-A154 (ribR::erm), available at the NBRP B. subtilis, Japan
BKE29300 (ribR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAAAGTCAGCCTCCTCT,  downstream forward: _UP4_GGATAGGGAAAGGAGCGGAA
BKK29300 (ribR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAAAGTCAGCCTCCTCT,  downstream forward: _UP4_GGATAGGGAAAGGAGCGGAA
References
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Time of last update: 2025-04-25 08:47:01

Author of last update: Melvin.boenninger