speD

speD
168

S-adenosylmethionine decarboxylase, required for biofilm formation

Locus
BSU_29010
Molecular weight
13.96 kDa
Isoelectric point
4.77
Protein length
126 aaSequenceBlast
Gene length
381 bpSequenceBlast
Function
spermidine, polyamine biosynthesis
Product
S-adenosylmethionine decarboxylase
Essential
no
E.C.
4.1.1.50
Synonyms
speD, ytcF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1586 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpN regulon

Gene
Coordinates
2,966,413 → 2,966,793
Phenotypes of a mutant
no biofilm formation PubMed
The protein
Catalyzed reaction/ biological activity
H+ + S-adenosyl-L-methionine --> CO2 + S-adenosyl-L-methioninamine (according to UniProt)
Protein family
prokaryotic AdoMetDC family (single member, according to UniProt)
Structure
1VR7 (PDB) (from Thermotoga maritima, 47% identity, 72% similarity)
O34426 (AlphaFold)
Expression and Regulation
Operons
Genes
gapB-speD
Description
PubMed
Regulation
gapB: repressed in the presence of glucose (CcpN) PubMed
Regulatory mechanism
CcpN: repression, PubMed, in ccpN regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
speD: the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic speD mRNA increases from 1.4 to 36 min) PubMed
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gapB→speD

2025-03-27 02:12:40

Jstuelk

105

FD19E158AAF06F7ED8F02CFEE19CA4A6483E5FCD

2E200F04B473223099E032055E071DE51C38D7C2

Genes
speD
Description
PubMed
Additional information
speD: the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic speD mRNA increases from 1.4 to 36 min) PubMed
Open in new tab

speD

2025-02-20 08:51:23

Jstuelk

71

5BB5A0EEC359A42ABEC36C427E4A396052596EDA

680CA8DB921CD01E59CC00D1733B359F825C21A8

Biological materials
Mutant
MGNA-A122 (ytcF::erm), available at the NBRP B. subtilis, Japan
BKE29010 (ΔspeD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT,  downstream forward: _UP4_TAAAGTAAATTGCGAACACA
BKK29010 (ΔspeD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT,  downstream forward: _UP4_TAAAGTAAATTGCGAACACA
GP2599 (ΔspeD::tet comIQ12L) (in DK1042), available in Jörg Stülke's lab
References
Loading

CDD5C7B1CE58352D29BB86BCC2C3685D1D8898ED

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Time of last update: 2025-04-09 21:27:55

Author of last update: Jstuelk