speD

speD
168

S-adenosylmethionine decarboxylase, required for biofilm formation

Locus
BSU_29010
Molecular weight
13.96 kDa
Isoelectric point
4.77
Protein length
126 aaSequenceBlast
Gene length
381 bpSequenceBlast
Function
spermidine, polyamine biosynthesis
Product
S-adenosylmethionine decarboxylase
Essential
no
E.C.
4.1.1.50
Synonyms
speD, ytcF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1586 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,966,413 → 2,966,793
Phenotypes of a mutant
no biofilm formation PubMed
The protein
Catalyzed reaction/ biological activity
H+ + S-adenosyl-L-methionine --> CO2 + S-adenosyl-L-methioninamine (according to UniProt)
Protein family
prokaryotic AdoMetDC family (single member, according to UniProt)
Structure
1VR7 (PDB) (from Thermotoga maritima, 47% identity, 72% similarity)
O34426 (AlphaFold)
Expression and Regulation
Operons
Genes
gapB-speD
Description
PubMed
Regulation
gapB: repressed in the presence of glucose (CcpN) PubMed
Regulatory mechanism
CcpN: repression, PubMed, in ccpN regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
speD: the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic speD mRNA increases from 1.4 to 36 min) PubMed
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gapB→speD

2025-03-27 02:12:40

Jstuelk

105

FD19E158AAF06F7ED8F02CFEE19CA4A6483E5FCD

2E200F04B473223099E032055E071DE51C38D7C2

Genes
speD
Description
PubMed
Additional information
speD: the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the monocistronic speD mRNA increases from 1.4 to 36 min) PubMed
Open in new tab

speD

2025-02-20 08:51:23

Jstuelk

71

5BB5A0EEC359A42ABEC36C427E4A396052596EDA

680CA8DB921CD01E59CC00D1733B359F825C21A8

Biological materials
Mutant
MGNA-A122 (ytcF::erm), available at the NBRP B. subtilis, Japan
BKE29010 (ΔspeD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT,  downstream forward: _UP4_TAAAGTAAATTGCGAACACA
BKK29010 (ΔspeD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTCATGGACCCCCCTT,  downstream forward: _UP4_TAAAGTAAATTGCGAACACA
GP2599 (ΔspeD::tet comIQ12L) (in DK1042), available in Jörg Stülke's lab
References
Loading

CDD5C7B1CE58352D29BB86BCC2C3685D1D8898ED

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Time of last update: 2025-04-10 03:39:29

Author of last update: Jstuelk