azlB

azlB
168

transcriptional repressor (Lrp family) of the azlB-azlC-azlD-brnQ-yrdK operon

Locus
BSU_26720
Molecular weight
17.32 kDa
Isoelectric point
4.87
Protein length
Gene length
Function
regulation of branched-chain amino acid transport and histidine export
Product
transcriptional repressor (Lrp family)
Essential
no
Synonyms
azlB, yrdG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1522 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,729,753 → 2,730,226
Phenotypes of a mutant
the inactivation of azlB confers resistance to otherwise toxic amino acids, such as 4-azaleucine, histidine, and asparagine and tolerance to 2,3-diaminopropionic acid due to resulting constitutive expression of the amino acid exporter AzlC-AzlD PubMed
The protein
Catalyzed reaction/ biological activity
binds to the promoter region of the azlB-azlC-azlD-brnQ-yrdK operon to repress its expression PubMed
Protein family
HTH asnC-type domain (aa 5-66) (according to UniProt)
Structure
2GQQ (PDB) (E. coli Lrp, 34% identity) PubMed
Effectors of protein activity
no inducer that triggers release of AzlB from the promoter DNA is known PubMed
Expression and Regulation
Operons
Description
Regulation
repressed by AzlB PubMed
Regulatory mechanism
AzlB: repression, PubMed, in azlB regulon
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azlByrdK

2025-02-21 00:14:27

Jstuelk

99

CE7A54721725B20F53C4F64C9153F7B05416307A

77D75B92D3B22CF1350DD9E108E868095EE55DEB

Biological materials
Mutant
GP3600(ΔazlB::spc trpC2), available in Jörg Stülke's lab PubMed
GP3601 (ΔazlB-azlC-azlD::kan) , available in Jörg Stülke's lab PubMed
GP3623 (ΔazlB-azlC-azlD::spec) , available in Jörg Stülke's lab PubMed
BKE26720 (ΔazlB::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTTCACAACCCCCAATAC,  downstream forward: _UP4_GATGAAATGTAGGAGGTTGT
BKK26720 (ΔazlB::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTTCACAACCCCCAATAC,  downstream forward: _UP4_GATGAAATGTAGGAGGTTGT
LacZ fusion
pGP3808 (in pAC7), available in Jörg Stülke's lab PubMed
References
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Time of last update: 2025-02-26 04:42:16

Author of last update: Jstuelk