yqhB

yqhB
168

similar to magnesium exporter, general stress protein, required for protection against oxidative stress

Locus
BSU_24750
Molecular weight
49.79 kDa
Isoelectric point
5.54
Protein length
442 aaSequenceBlast
Gene length
1329 bpSequenceBlast
Function
protection against stress conditions
Product
unknown
Essential
no
Synonyms
yqhB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,561,585 → 2,562,913
The protein
Protein family
UPF0053 family(according to UniProt)
Domains
CNNM transmembrane domain (aa 7-210) (according to UniProt)
DUF21 domain (14-210)
2 CBS domains (aa 229-290, aa 295-355) (according to UniProt)
Structure
4HG0 (PDB) (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)
P54505 (AlphaFold)
Paralogous protein(s)
YrkA, YugS, YhdT, MpfA
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
yqhB
Description
PubMed
Regulation
induced by stress (SigB) PubMed
Regulatory mechanism
LexA: repression, PubMed, in lexA regulon
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
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yqhB

2025-04-03 05:22:40

ghost

61

aa802dcb866e1b8bfe88add8a5dd09009d796528

D4EB08D8CC6DF167D7839EFE03FE502094F3FEAA

Biological materials
Mutant
MGNA-C474 (yqhB::erm), available at the NBRP B. subtilis, Japan
BKE24750 (ΔyqhB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA,  downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC
BKK24750 (ΔyqhB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCACCTCCCTTTTCCGA,  downstream forward: _UP4_TAGCCCGTGAAAGGCTGTTC
Expression vectors
pGP2935 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3467 (223-359aa) (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3472 (223-359aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
References
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E53B8D437B848B637AEC8C1BB9428EC8B6EB280E

Page visits: 4620

Time of last update: 2025-04-13 10:18:54

Author of last update: LKrueger