mleN

mleN
168

malate-H+/Na+-lactate antiporter, uptake of D-asparagine

Locus
BSU_23560
Molecular weight
50.05 kDa
Isoelectric point
7.25
Protein length
468 aaSequenceBlast
Gene length
1407 bpSequenceBlast
Function
malate uptake
Product
malate-H+/Na+-lactate antiporter
Essential
no
Synonyms
mleN, yqkI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1757 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,452,800  2,454,206
Phenotypes of a mutant
resistant to otherwise toxic D-asparagine PubMed
abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present PubMed
The protein
Catalyzed reaction/ biological activity
uptake of D-asparagine PubMed
Protein family
NhaC Na(+)/H(+) (TC 2.A.35) antiporter family (with NhaC, according to UniProt)
Structure
P54571 (AlphaFold)
Paralogous protein(s)
NhaC
Localization
membrane associated PubMed
Expression and Regulation
Operons
Genes
mleN-mleA
Description
PubMed
Regulation
''ansA'': expressed in the presence of asparagine (AnsR) PubMed
Regulatory mechanism
AnsR: repression, PubMed, in ansR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mleN→mleA

2025-03-09 00:58:59

ghost

73

2adb475a1d359d8484e1d13c3462a134569ab20a

12E51CE78001847EEC34BF1A5209896CF1E02F0D

Genes
ansA-ansB-mleN-mleA
Description
PubMed
Regulation
AnsA: expressed in the presence of asparagine (AnsR) PubMed
Expression
expressed in the presence of asparagine (AnsR) PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
AnsR: transcription repression, PubMed, in ansR regulon
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ansA→mleA

2025-04-04 08:53:37

Jstuelk

102

6E6CC6ADE2F20EBE93C9F1ED4C2EF4E87AF126B1

D7F0E184034763E326DE2841E35068D2FD73DD5D

Biological materials
Mutant
GP1460 (ΔmleN::kan), available in  Jörg Stülke's lab PubMed
GP4165 (ΔmleN::kan), available in  Jörg Stülke's lab
MGNA-C411 (yqkI::erm), available at the NBRP B. subtilis, Japan
GP1460 (mleN::spc), available in Jörg Stülke's lab
BKE23560 (mleN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT,  downstream forward: _UP4_GGCTAATAGGGGAGGATCAC
BKK23560 (mleN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT,  downstream forward: _UP4_GGCTAATAGGGGAGGATCAC
LacZ fusion
pGP388 (in pAC7), available in Jörg Stülke's lab PubMed
References
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49DF23B8E19658DB32835B6D23DD21E4BF481B20

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Time of last update: 2025-04-05 22:43:35

Author of last update: Jstuelk