Difference between revisions of "CdaS"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19430&redirect=T BSU19430] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU19430&redirect=T BSU19430] | ||
* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2FB5 2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity) | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=2FB5 2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity) | ||
Revision as of 13:55, 2 April 2014
- Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
| Gene name | cdaS |
| Synonyms | yojJ |
| Essential | no |
| Product | sporulation-specific diadenylate cyclase |
| Function | synthesis of c-di-AMP |
| Gene expression levels in SubtiExpress: cdaS | |
| MW, pI | 22 kDa, 5.367 |
| Gene length, protein length | 621 bp, 207 aa |
| Immediate neighbours | yojK, yojI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU19430
Phenotypes of a mutant
Database entries
- BsubCyc: BSU19430
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family:
- Paralogous protein(s): CdaA
Extended information on the protein
- Kinetic information:
- Domains:
- contains a DAC domain involved in the synthesis of c-di-AMP PubMed
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU19430
- Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)
- UniProt: O31854
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP983 (cdaS::ermC), available in Jörg Stülke's lab
- GP1360 (cdaS::spc), available in Jörg Stülke's lab
- Expression vector:
- IPTG inducible expression of B. cereus Strep-cdaS in E. coli: pGP2593 (in pGP172), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- His-tag fusion:
- pGP2591 10xHis-cdaS under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
- pGP2592 10xHis-cdaS-L44F under the control of native promoter (based on pAC7), available in Jörg Stülke's lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
