ynbA

ynbA
168

GTP-binding protein, ribosome-splitting factor, rescues translationally arrested ribosomes

Locus
BSU_17430
Molecular weight
37.51 kDa
Isoelectric point
9.24
Protein length
420 aaSequenceBlast
Gene length
1263 bpSequenceBlast
Function
rescuing of translationally arrested ribosomes
Product
GTP-binding protein, ribosome-splitting factor
Essential
no
Synonyms
ynbA, hflX
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2262 (Galperin et al., 2021)

Gene
Coordinates
1,875,304  1,876,566
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes GTP and readily exchanges GDP for GTP
rescuing of translationally arrested ribosomes PubMed
Protein family
TRAFAC class OBG-HflX-like GTPase superfamily (with Obg and YyaF, according to UniProt)
Domains
Hflx-type G domain (aa 201-363) (according to UniProt)
Structure
5ADY (PDB) (the protein from E. coli in complex with the ribosome, 41% identity) PubMed
P94478 (AlphaFold)
Effectors of protein activity
binds ppGpp PubMed, ppGpp may inhibit activity PubMed
Paralogous protein(s)
EngA, YyaF, Era, YsxC, RbgA, ThdF, Obg, YqeH
Expression and Regulation
Operons
Genes
ynbA-ynbB-glnR-glnA
Description
PubMed
Regulation
the mRNA is processed between glnR and glnA by RNase Y, this requires the RicA-RicF-RicT complex PubMed
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ynbA→glnA

2025-03-17 13:31:01

Jstuelk

292

d45811f6bc7bef80a041d78aaa209f36dccdee6a

2408FECBCAE7255480B230470AF58DDA9F084F5A

Biological materials
Mutant
MGNA-A020 (ynbA::erm), available at the NBRP B. subtilis, Japan
BKE17430 (ynbA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT,  downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
BKK17430 (ynbA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT,  downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
References
Reviews
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Original Publications
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9F6B78C1932FB56F7F71430DB16BC862A312407B

Page visits: 3117

Time of last update: 2025-04-26 12:50:34

Author of last update: Robert.warneke