truB

truB
168

tRNA pseudouridine 55 synthase

Locus
BSU_16660
Molecular weight
34.05 kDa
Isoelectric point
6.65
Protein length
309 aaSequenceBlast
Gene length
930 bpSequenceBlast
Function
tRNA modification
Product
tRNA pseudouridine 55 synthase
Essential
no
Synonyms
truB, ylxN, ymxF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0130 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,736,886  1,737,815
The protein
Catalyzed reaction/ biological activity
uridine55 in tRNA --> pseudouridine55 in tRNA (according to UniProt)
Protein family
pseudouridine synthase TruB family (single member, according to UniProt)
Structure
1ZE1 (PDB) (from Thermotoga maritima, 36% identity) PubMed
P32732 (AlphaFold)
Biological materials
Mutant
BKE16660 (truB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCCTTTCACTCCTTC,  downstream forward: _UP4_CAATAGAAAAAAGGTGACCG
BKK16660 (truB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCCTTTCACTCCTTC,  downstream forward: _UP4_CAATAGAAAAAAGGTGACCG
References
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Time of last update: 2025-04-24 23:15:02

Author of last update: Jstuelk