rfaB

rfaB
168

similar to alkaline-shock protein

Locus
BSU_15830
Molecular weight
13.05 kDa
Isoelectric point
7.08
Protein length
120 aaSequenceBlast
Gene length
363 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
yloU
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1302 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,656,064  1,656,426
The protein
Protein family
asp23 family (with RfaA , according to UniProt)
Structure
O34318 (AlphaFold)
Paralogous protein(s)
RfaA
Expression and Regulation
Operons
Genes
rfaB-fakA
Description
PubMed
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rfaB→fakA

2025-02-15 15:41:43

Bzhu

85

59df383c358e285f8f394d9d07619671e9b5d639

60743BCE1ECE1AB944532A871551DEAAB9A8E78C

Biological materials
Mutant
MGNA-B375 (yloU::erm), available at the NBRP B. subtilis, Japan
GP1465 (yloU-cat ), available in Jörg Stülke's lab PubMed
GP1467 (yloU-fakA-cat ), available in Jörg Stülke's lab PubMed
BKE15830 (rfaB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA,  downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT
BKK15830 (rfaB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA,  downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT
Expression vectors
GP1476 (chromosomal yloU-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
pGP1330 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP1324 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
pGP3620: expression of Strep-rfaB by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3621: expression of rfaB-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3629 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
pGP3638: expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab
pGP3645 (N-terminal GST-Tag, purification from E. coli, in pGEX-6P-1), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP3792 (spc, based on pGP1331), available in Jörg Stülke's lab
GFP fusion
GP1472 (spc, based on pBP43), available in Jörg Stülke's lab PubMed
References
Reviews
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Original Publications
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Time of last update: 2025-04-03 10:20:34

Author of last update: Dennis.wicke