ylmH

ylmH
168

ribosome-associated quality control factor, stabilizes the P-site tRNA conformation, processivity factor essential for poly-alanine synthesis during ribosome quality control elongation by RqcH, redundant with RqcP

Locus
BSU_15410
Molecular weight
28.92 kDa
Isoelectric point
7.04
Protein length
257 aaSequenceBlast
Gene length
774 bpSequenceBlast
Function
ribosome-associated quality control
Product
ribosome-associated quality control factor
Essential
no
Synonyms
ylmH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2302 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,611,654 → 1,612,427
The protein
Catalyzed reaction/ biological activity
binds to the 50S ribosome subunit via 23S rRNA PubMed
binds peptidyl-tRNA-50S complexes in a position analogous to that of S4-domain-containing protein RqcP PubMed
Protein family
S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
Domains
S4 RNA-binding domain (aa 181-251) PubMed
Structure
8S1P (PDB) YlmH bound to the 50S ribosome subunit PubMed
8S1U (PDB) YlmH and RqcH bound to the 50S ribosome subunit PubMed
2FPH (PDB) (from Streptococcus pneumoniae, N-terminal domain, corresponds to aa 4 ... 158, 32% identity)
P71020 (AlphaFold)
Expression and Regulation
Operons
Genes
ylmD-ylmE-sepF-ylmG-ylmH
Description
PubMed
Regulation
repressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: repression, PubMed, in spo0A regulon
Open in new tab

ylmD→ylmH

2025-03-16 19:12:27

ghost

123

d444960f93629c61035f6c91fd3ada83c687ea2d

12299F7737A00124F5303235F9BC3A294A212ACF

Biological materials
Mutant
MGNA-B364 (ylmH::erm), available at the NBRP B. subtilis, Japan
BKE15410 (ΔylmH::erm trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTCTTTCCCTTCTATG,  downstream forward: _UP4_TAGTCGGTTTTTCAGTTTTT
BKK15410 (ΔylmH::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTCTTTCCCTTCTATG,  downstream forward: _UP4_TAGTCGGTTTTTCAGTTTTT
References
Loading

BD5ACF930DD63E258D71569326752C9A1D7B9324

Page visits: 3211

Time of last update: 2025-04-03 01:02:30

Author of last update: Jstuelk