ogt

ogt
168

O6-methylguanine DNA alkyltransferase

Locus
BSU_13540
Molecular weight
18.61 kDa
Isoelectric point
7.09
Protein length
165 aaSequenceBlast
Gene length
498 bpSequenceBlast
Function
DNA repair
Product
O6-methylguanine DNA alkyltransferase
Essential
no
E.C.
2.1.1.63
Synonyms
ogt, dat
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0350 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,421,426  1,421,923
The protein
Catalyzed reaction/ biological activity
6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] --> 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] --> thymidine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
Protein family
MGMT family (with AdaB, according to UniProt)
Structure
1SFE (PDB) (from E. coli, 36% identity, 51% similarity) PubMed
P11742 (AlphaFold)
Expression and Regulation
Operons
Genes
kinE-ogt
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

kinE→ogt

2025-03-15 07:58:25

ghost

117

63e752d8925a0480f853181e8e69a5b2e969362b

4B7DD3F359520D4DAAB003D0DC6AF3C2D719EB36

Biological materials
Mutant
BKE13540 (ogt::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT,  downstream forward: _UP4_TAAAACATATGGCACGTTCC
BKK13540 (ogt::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTTTCGGCTGTCGTATAGT,  downstream forward: _UP4_TAAAACATATGGCACGTTCC
References
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Page visits: 2430

Time of last update: 2025-04-09 21:47:10

Author of last update: Melvin.boenninger