Difference between revisions of "SpoIISA"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU12830&redirect=T BSU12830] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
| Line 98: | Line 99: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU12830&redirect=T BSU12830] | ||
* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3O6Q 3O6Q] (cytoplasmic fragment of [[SpoIISA]] (CSpoIISA) in complex with [[SpoIISB]]) {{PubMed|21147767}} | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3O6Q 3O6Q] (cytoplasmic fragment of [[SpoIISA]] (CSpoIISA) in complex with [[SpoIISB]]) {{PubMed|21147767}} | ||
Revision as of 13:30, 2 April 2014
- Description: lethal when synthesized during vegetative growth in the absence of SpoIISB
| Gene name | spoIISA |
| Synonyms | ykaC |
| Essential | no |
| Product | toxin |
| Function | programmed cell death |
| Gene expression levels in SubtiExpress: spoIISA | |
| Interactions involving this protein in SubtInteract: SpoIISA | |
| MW, pI | 28 kDa, 4.683 |
| Gene length, protein length | 744 bp, 248 aa |
| Immediate neighbours | spoIISB, pit |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
sporulation proteins, toxins, antitoxins and immunity against toxins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU12830
Phenotypes of a mutant
Database entries
- BsubCyc: BSU12830
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: three putative membrane-spanning segments and a cytoplasmic domain
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane PubMed
Database entries
- BsubCyc: BSU12830
- UniProt: O34853
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- levels increase at the point of entry into stationary phase of cell cultures grown in sporulation-inducing medium, this is independent of Spo0A PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767]
[WorldCat.org]
[DOI]
(I p)
Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016]
[WorldCat.org]
[DOI]
(P p)
E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520]
[WorldCat.org]
[DOI]
(P p)
Additional Publications: PubMed
