yitU

yitU
168

5'-nucleotidase, dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)

Locus
BSU_11140
Molecular weight
30.48 kDa
Isoelectric point
4.67
Protein length
Gene length
Function
dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)
Product
5'-nucleotidase
Essential
no
Synonyms
yitU

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,190,490  1,191,302
The protein
Catalyzed reaction/ biological activity
dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity) PubMed
5-amino-6-(5-phospho-D-ribitylamino)uracil + H2O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)
Protein family
HAD superfamily (according to UniProt) PubMed
Cof family (according to UniProt)
Structure
1RKQ (PDB) (YidA from E. coli, 31% identity)
Expression and Regulation
Operons
Genes
Description
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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yitUyizC

2025-01-10 14:02:00

Jstuelk

91

91a882259e9ce11501fff2dd8d76b5cf29596bee

40A008BA43DB09D5A877D364110175189F81FE3C

Biological materials
Mutant
MGNA-B185 (yitU::erm), available at the NBRP B. subtilis, Japan
BKE11140 (yitU::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGAAAAGCTCCTTTAACC,  downstream forward: _UP4_TAAAGAAGGTCCGTATTAAT
BKK11140 (yitU::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGAAAAGCTCCTTTAACC,  downstream forward: _UP4_TAAAGAAGGTCCGTATTAAT
References
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Time of last update: 2025-01-14 15:43:24

Author of last update: Jstuelk