yhcT

yhcT
168

pseudouridylate synthase, involved in pseudouridylation of 23S rRNA positions 1940, 1944, and 1946

Locus
BSU_09210
Molecular weight
33.59 kDa
Isoelectric point
9.64
Protein length
302 aaSequenceBlast
Gene length
909 bpSequenceBlast
Function
23S rRNA modification
Product
putative sporulation-specific pseudouridylate synthase
Essential
no
Synonyms
yhcT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
995,624  996,532
The protein
Catalyzed reaction/ biological activity
uridine in RNA --> ψ-uridine in 23S rRNA at positions 1940, 1944, and 1946 PubMed
Protein family
S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RluA family (with YjbO and RluD, according to UniProt)
Domains
S4 RNA-binding domain (aa 19-90) (according to UniProt)
Structure
1V9F (PDB) (RluD from E. coli, 36% identical) PubMed
P54604 (AlphaFold)
Paralogous protein(s)
YjbO, RluD
Expression and Regulation
Operons
Genes
S319-yhcT
Description
PubMed
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S319→yhcT

2025-04-06 18:06:27

Jstuelk

68

6f64a0d8c081c26af4c184a25a1a1f324bcb793e

1FD2EC4F2F439B7179C5E952240E79C51A16A2C1

Biological materials
Mutant
MGNA-A666 (yhcT::erm), available at the NBRP B. subtilis, Japan
BKE09210 (yhcT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTAAATGGCTCCTTTT,  downstream forward: _UP4_TGAAGAAAAGCCGCAGGCAC
BKK09210 (yhcT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTAAATGGCTCCTTTT,  downstream forward: _UP4_TGAAGAAAAGCCGCAGGCAC
References
Research papers
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064AE8CE6D52E35582F90654AA9548963573C05C

Page visits: 2426

Time of last update: 2025-04-06 16:22:36

Author of last update: Jstuelk