ThiC

• Description: biosynthesis of the pyrimidine moiety of thiamine
  
  

Gene name	thiC
Synonyms	thiA
Essential	no
Product	unknown
Function	biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiC
Metabolic function and regulation of this protein in SubtiPathways: thiC
MW, pI	65 kDa, 5.262
Gene length, protein length	1770 bp, 590 aa
Immediate neighbours	ygaJ, ygaK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU08790

Database entries

• BsubCyc: BSU08790

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: thiC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-556 PubMed
  • phosphorylated on Ser-565, Ser-586 and Tyr-589 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU08790

• Structure: 1G6C

• UniProt: P45740

• KEGG entry: [2]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: thiC PubMed

• Expression browser: thiC PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine and 2-methyl-4-amino-5-hydroxymethylpyrimidine PubMed
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:
  • 1A603 ( thiC::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)