citM

citM
168

Mg2+-citrate transporter

Locus
BSU_07610
Molecular weight
45.67 kDa
Isoelectric point
6.23
Protein length
433 aaSequenceBlast
Gene length
1302 bpSequenceBlast
Function
uptake of citrate and magnesium
Product
Mg2+-citrate transporter
Essential
no
Synonyms
citM, yflO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2851 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, CitT regulon

Gene
Coordinates
834,383 → 835,684
Phenotypes of a mutant
no growth with citrate as sole carbon source PubMed
The protein
Protein family
CitM transporter family (TC 2.A.11) (according to UniProt)
Structure
P55069 (AlphaFold)
Paralogous protein(s)
CitH
Localization
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
citM-yflN
Description
PubMed
Regulation
induced by citrate (CitT) PubMed
Regulatory mechanism
CitT: activation, PubMed, in citT regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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citM→yflN

2025-03-10 07:07:49

ghost

77

3c6c67bfa157ec679e863c4c5073c923f5b317fb

F07F80A43DA7CF191444130E860CCF37E8CB2FF5

Biological materials
Mutant
MGNA-C252 (citM::erm), available at the NBRP B. subtilis, Japan
GP2244 (Δ''citM::ermC''), available in Jörg Stülke's lab
GP2760 (Δ''citM::kan''), available in Jörg Stülke's lab
GP2761 (Δ''citM::spec''), available in Jörg Stülke's lab
BKE07610 (ΔcitM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCCATTCCCCCTTAAA,  downstream forward: _UP4_TGATAATTCAGGCGTAATGA
BKK07610 (ΔcitM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCCATTCCCCCTTAAA,  downstream forward: _UP4_TGATAATTCAGGCGTAATGA
GP2763 (Δ''citM::kan Δ''''mgtE::spec''), available in Jörg Stülke's lab
FLAG-tag construct
GP2767 citM-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
LacZ fusion
pGP2965 (in pAC6), available in Jörg Stülke's lab
References
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C0DED68802EF78D2BC16507234AAC3B60D236E68

Page visits: 5535

Time of last update: 2025-04-06 17:46:54

Author of last update: LKrueger