iolT

iolT
168

major transporter for inositol

Locus
BSU_06230
Molecular weight
51.47 kDa
Isoelectric point
9.57
Protein length
473 aaSequenceBlast
Gene length
1422 bpSequenceBlast
Function
myo-inositol uptake
Product
major transporter for inositol
Essential
no
Synonyms
iolT, ydjK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2814 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
676,442  677,863
The protein
Catalyzed reaction/ biological activity
transport of myo-inositol PubMed
Protein family
major facilitator superfamily (according to UniProt)
Sugar transporter (TC 2.A.1.1) family (according to UniProt)
Structure
4LDS (PDB) (glucose transporter from Staphylococcus epidermidis, 36% identity) PubMed
O34718 (AlphaFold)
Paralogous protein(s)
IolT, AraE, YwtG, YfiG, YncC, CsbC
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
iolT
Description
PubMed
Regulation
induced in the presence of inositol (IolR) PubMed
Regulatory mechanism
IolR: repression, PubMed, in iolR regulon
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iolT

2025-03-29 07:59:20

Bzhu

64

6a1338438d535d68dd072e0d79d846b1882bfdca

67755B298561AD189D7FCF9405D6F36C3149DFD4

Biological materials
Mutant
MGNA-C218 (ydjK::erm), available at the NBRP B. subtilis, Japan
BKE06230 (iolT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTGTTTCCCCCAGTCT,  downstream forward: _UP4_TAATTTAAAAAAGAATCCGC
BKK06230 (iolT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTGTTTCCCCCAGTCT,  downstream forward: _UP4_TAATTTAAAAAAGAATCCGC
References
Tanaka K, Natsume A, Ishikawa S, Takenaka S, Yoshida KI A new-generation of Bacillus subtilis cell factory for further elevated scyllo-inositol production. Microbial cell factories. 2017 Apr 21; 16(1):67. doi:10.1186/s12934-017-0682-0. PMID:28431560
Iancu CV, Zamoon J, Woo SB, Aleshin A, Choe JY Crystal structure of a glucose/H+ symporter and its mechanism of action. Proceedings of the National Academy of Sciences of the United States of America. 2013 Oct 29; 110(44):17862-7. doi:10.1073/pnas.1311485110. PMID:24127585
Morinaga T, Matsuse T, Ashida H, Yoshida K Differential substrate specificity of two inositol transporters of Bacillus subtilis. Bioscience, biotechnology, and biochemistry. 2010; 74(6):1312-4. . PMID:20530884
Yoshida K, Yamamoto Y, Omae K, Yamamoto M, Fujita Y Identification of two myo-inositol transporter genes of Bacillus subtilis. Journal of bacteriology. 2002 Feb; 184(4):983-91. . PMID:11807058

4A77316AA94F9C11DB70AB76711AACBD28098AA1

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Time of last update: 2025-04-07 00:36:29

Author of last update: Melvin.boenninger