ydaK

ydaK
168

general stress protein, may act as c-di-GMP receptor, required for extracellular polysaccharide synthesis by YdaL-YdaM-YdaN

Locus
BSU_04280
Molecular weight
32.53 kDa
Isoelectric point
6.7
Protein length
283 aaSequenceBlast
Gene length
852 bpSequenceBlast
Function
unknown
Product
putative c-di-GMP receptor protein, general stress protein
Essential
no
Synonyms
ydaK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2199 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
480,013  480,864
The protein
Domains
four transmembrane helices at the N-terminus, they are essential for activation of EPS formation by YdaL, YdaM, and YdaN PubMed
contains a C-terminal degenerated GGDEF domain (aa 162-283) PubMed
Cofactors
binds c-di-GPM at high concentration (K(D) for c-di-GMP: 1.1 myM) PubMed
Structure
P96585 (AlphaFold)
Localization
cell membrane, forms clusters at the cell poles and septa (with YdaM and YdaN) PubMed
Expression and Regulation
Operons
Genes
ydaJ-ydaK-ydaL-ydaM-ydaN
Description
PubMed
Regulation
expressed during stress (SigB) PubMed
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
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ydaJ→ydaN

2025-04-04 07:43:16

Jstuelk

90

b605f66f599d67e539c67d5e096567c2b2e56ae4

80FCF67C03206CFA39A835FC21447D9DDEAF08EF

Biological materials
Mutant
MGNA-C074 (ydaK::erm), available at the NBRP B. subtilis, Japan
GP1592 (kan) available in Jörg Stülke's lab
JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
BKE04280 (ydaK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAATAAGCAAATAATTTTT,  downstream forward: _UP4_ATGAATGAACTATAAAGGAA
BKK04280 (ydaK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAATAAGCAAATAATTTTT,  downstream forward: _UP4_ATGAATGAACTATAAAGGAA
References
Reviews
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Original Publications
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70ED3CE683F3A3F785480F40986CABE7729C17C7

Page visits: 3944

Time of last update: 2025-04-06 18:31:27

Author of last update: Jstuelk