lmrB

lmrB
168

lincomycin-resistance protein (multidrug resistance pump)

Locus
BSU_02670
Molecular weight
51.54 kDa
Isoelectric point
9.7
Protein length
479 aaSequenceBlast
Gene length
1440 bpSequenceBlast
Function
resistance to lincomycin
Product
lincomycin-resistance protein
Essential
no
Synonyms
lmrB, yccA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2814 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, LmrA regulon

Gene
Coordinates
288,653  290,092
The protein
Protein family
major facilitator superfamily (according to UniProt)
EmrB family (according to UniProt)
Structure
O35018 (AlphaFold)
Paralogous protein(s)
YhcA, YcnB
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
lmrA-lmrB
Description
PubMed
Regulation
induced by flavonoids such as quercetin (LmrA)PubMed
Regulatory mechanism
LmrA: repression, PubMed, in lmrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
Open in new tab

lmrA→lmrB

2025-04-06 20:43:13

ghost

114

be7795d98f26be96a984dc95802c21f2dcb1e545

FCA0758D41C878E1059BF369D1C30C97AACB6B0C

Biological materials
Mutant
GP1701 (''lmrB::aphA3''), available in Jörg Stülke's lab
GP1703 (''lmrB::aphA3 ycnB::spc''), available in Jörg Stülke's lab
BKE02670 (lmrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCCCTCTCTATCAAC,  downstream forward: _UP4_TAATATGAAAAGCCCCTGAC
BKK02670 (lmrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCCCTCTCTATCAAC,  downstream forward: _UP4_TAATATGAAAAGCCCCTGAC
References
Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. Molecular microbiology. 2011 Sep; 81(6):1459-73. doi:10.1111/j.1365-2958.2011.07777.x. PMID:21815947
Hirooka K, Kunikane S, Matsuoka H, Yoshida K, Kumamoto K, Tojo S, Fujita Y Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids. Journal of bacteriology. 2007 Jul; 189(14):5170-82. . PMID:17483215
Yoshida K, Ohki YH, Murata M, Kinehara M, Matsuoka H, Satomura T, Ohki R, Kumano M, Yamane K, Fujita Y Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH. Journal of bacteriology. 2004 Sep; 186(17):5640-8. . PMID:15317768
Kumano M, Fujita M, Nakamura K, Murata M, Ohki R, Yamane K Lincomycin resistance mutations in two regions immediately downstream of the -10 region of lmr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants. Antimicrobial agents and chemotherapy. 2003 Jan; 47(1):432-5. . PMID:12499232

23483430AE0381406EF2349A18CFAE1F14F9B180

Page visits: 5525

Time of last update: 2025-04-07 09:09:45

Author of last update: Melvin.boenninger