ycbF

ycbF
168

glucarate dehydratase

Locus
BSU_02490
Molecular weight
50.62 kDa
Isoelectric point
5.59
Protein length
455 aaSequenceBlast
Gene length
1368 bpSequenceBlast
Function
glucarate utilization
Product
glucarate dehydratase
Essential
no
E.C.
4.2.1.40
Synonyms
ycbF, gudD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4948 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
271,800  273,167
The protein
Catalyzed reaction/ biological activity
D-glucarate --> 5-dehydro-4-deoxy-D-glucarate + H2O (according to UniProt)
Protein family
mandelate racemase/muconate lactonizing enzyme family (according to UniProt)
Structure
1EC7 (PDB) (from E. coli, 73% identity) PubMed
P42238 (AlphaFold)
Expression and Regulation
Operons
Genes
ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ
Description
PubMed
Regulation
induced by D-glucarate/galactarate (YcbG) PubMed
Regulatory mechanism
YcbG: repression, PubMed, in ycbG regulon
AbrB: repression, PubMed, in abrB regulon
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ycbC→ycbJ

2025-03-10 23:53:20

ghost

122

a8e395a36da4d417bafa3e16f332fbbf2dcf2e61

24EBFC92FB6A93BD115AC81FF68694FB539EE76E

Biological materials
Mutant
MGNA-C029 (ycbF::erm), available at the NBRP B. subtilis, Japan
BKE02490 (ycbF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCATTTTCCTCCCTTTA,  downstream forward: _UP4_TAAGTTTGATTGTAAATATT
BKK02490 (ycbF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCATTTTCCTCCCTTTA,  downstream forward: _UP4_TAAGTTTGATTGTAAATATT
References
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372F2A420309DB0193AEF36A740EBA26410F97F2

Page visits: 1959

Time of last update: 2025-04-10 13:37:13

Author of last update: Melvin.boenninger