skfF
168

ABC transporter (permease), export of the spore killing factor

Locus

BSU_01960

Molecular weight

51.72 kDa

Isoelectric point

9.22

Protein length

448 aaSequenceBlast

Gene length

1344 bpSequenceBlast

Function

export of the spore killing factor SkfA

Product

ABC transporter (permease)

Essential

Synonyms

skfF, ybdB

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene

Coordinates

217,697 → 219,040

Phenotypes of a mutant

inactivation of ''skfF'' reduces sporulation efficiency to 62.3% that of wild type cells PubMed

The protein

Structure

O31428 (AlphaFold)

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes

skfA-skfB-skfC-skfE-skfF-skfG-skfH

Description

PubMed

Regulation

expressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism

Spo0A: activation, PubMed, in spo0A regulon

AbrB: repression, PubMed, in abrB regulon

PhoP: activation, PubMed, in phoP regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Additional information

Northern blotting during during phosphate limitation showed an intense 0.25 kb 'skfA'-specific transcript, and a weaker 6.5 kb 'skfA-skfB-skfC-skfE-skfF-skfG-skfH' transcript.

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Biological materials

Mutant

MGNA-C516 (ybdB::erm), available at the NBRP B. subtilis, Japan

BKE01960 (ΔskfF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTTTCCTGACCTT, downstream forward: _UP4_TAGTGGTTATGTAGAGTTAT

BKK01960 (ΔskfF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTTTTTCCTGACCTT, downstream forward: _UP4_TAGTGGTTATGTAGAGTTAT

References

Reviews

Original Publications

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Time of last update: 2025-04-06 15:17:23

Author of last update: Jstuelk

skfF 168

skfF
168