dusB1

dusB1
168

major tRNA-dihydrouridine synthase B

Locus
BSU_00810
Molecular weight
36.91 kDa
Isoelectric point
7.87
Protein length
333 aaSequenceBlast
Gene length
1002 bpSequenceBlast
Function
tRNA maturation
Product
major tRNA-dihydrouridine synthase B
Essential
no
Synonyms
yacF, dusB, dus1
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0042 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
87,634 → 88,635
The protein
Catalyzed reaction/ biological activity
5,6-dihydrouridine in tRNA + NAD+ --> uridine in tRNA + H+ + NADH PubMed
5,6-dihydrouridine in tRNA + NADP+ --> uridine in tRNA + H+ + NADPH PubMed
Protein family
Dus family (with DusB2, according to UniProt)
Cofactors
FMN PubMed
Structure
6EI9 (PDB) (from E. coli, 39.5% identity) PubMed
1VHN (PDB) (from Thermotoga maritima, 35% identity, 54% similarity) PubMed
P37567 (AlphaFold)
Paralogous protein(s)
DusB2
Expression and Regulation
Operons
Genes
pabB-pabA-pabC-sul-folB-folK-yazB-dusB1-lysS
Description
PubMed
Regulation
translation repressed by tryptophan (MtrB) PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pabB→lysS

2025-04-04 11:44:49

Jstuelk

121

8c465dd3eec1aaa32d5e91206834f9bb99ef474a

7B9041CE94396BF42D285F7C0EDD3A2544761EEE

Additional information
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
MGNA-B927 (yacF::erm), available at the NBRP B. subtilis, Japan
BKE00810 (ΔdusB1::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA,  downstream forward: _UP4_TAATACTCACCTCTATTTGC
BKK00810 (ΔdusB1::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTTTTCTCCTCCTTTCA,  downstream forward: _UP4_TAATACTCACCTCTATTTGC
References
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Time of last update: 2025-04-10 01:26:03

Author of last update: Jstuelk