yaaJ

yaaJ
168

tRNA specific adenosine deaminase

Locus
BSU_00180
Molecular weight
17.61 kDa
Isoelectric point
5.64
Protein length
161 aaSequenceBlast
Gene length
486 bpSequenceBlast
Function
tRNA modification
Product
tRNA specific adenosine deaminase
Essential
no
Synonyms
yaaJ, tadA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0590 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
25,852  26,337
The protein
Catalyzed reaction/ biological activity
Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2) (according to UniProt)
adenosine34 in tRNA + H+ + H2O --> inosine34 in tRNA + NH4+ (according to UniProt)
Protein family
Cytidine and deoxycytidylate deaminase family (according to UniProt)
Domains
CMP/dCMP-type deaminase domain (aa 2-120) (according to UniProt)
Structure
7CPH (PDB) PubMed
2B3J (PDB) (the protein of Staphylococcus aureus, complex with RNA, 61.7% identity) PubMed
P21335 (AlphaFold)
Expression and Regulation
Operons
Genes
yaaJ-scr
Description
null
Sigma factors
SigA: sigma factor, (both promoters), in sigA regulon
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yaaJ→scr

2025-04-04 12:49:39

ghost

122

b2d28cf402e740ffdb8d72c8cd99dd7c5f187533

7A6D368CD211CDB194D1980F4321F881B55AD15E

Biological materials
Mutant
MGNA-B891 (yaaJ::erm), available at the NBRP B. subtilis, Japan
BKE00180 (yaaJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT,  downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
BKK00180 (yaaJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT,  downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
References
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5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB

Page visits: 4823

Time of last update: 2025-04-14 22:41:25

Author of last update: Jstuelk