MurAB

• Description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  
  

Gene name	murAB
Synonyms	murZ, lssF, rev-4
Essential	no
Product	UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murAB
Metabolic function and regulation of this protein in SubtiPathways: murAB
MW, pI	45 kDa, 5.831
Gene length, protein length	1287 bp, 429 aa
Immediate neighbours	glpX, ywjH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components

The gene

Basic information

• Locus tag: BSU37100

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)

• Protein family: MurA subfamily (according to Swiss-Prot)

• Paralogous protein(s): MurAA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3SG1 (from B. anthracis, 50% identity, 81% similarity)

• UniProt: P19670

• KEGG entry: [2]

• E.C. number: 2.5.1.7

Expression and regulation

• Operon: murAB-glpX PubMed

• Expression browser: murAB PubMed

• Sigma factor:

• Regulation: constitutive PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • pGP2595: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ankur Gautam, Praveen Rishi, Rupinder Tewari
UDP-N-acetylglucosamine enolpyruvyl transferase as a potential target for antibacterial chemotherapy: recent developments.
Appl Microbiol Biotechnol: 2011, 92(2);211-25
[PubMed:21822642] [WorldCat.org] [DOI] (I p)