Difference between revisions of "PGP886"

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(Created page with "250px|thumb|right|'''pGP886: click to enlarge''' The vector was constructed in Jörg Stülke's lab and it is suitable for the xylose-inducible expression...")
 
 
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*An additional base has to be inserted in the forward primer to restore the frame.
 
*An additional base has to be inserted in the forward primer to restore the frame.
 
*Don't forget a stop codon in the reverse primer.
 
*Don't forget a stop codon in the reverse primer.
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<pubmed>24493247</pubmed>
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==  Return to [[Plasmids]] ==

Latest revision as of 15:26, 6 February 2014

pGP886: click to enlarge

The vector was constructed in Jörg Stülke's lab and it is suitable for the xylose-inducible expression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin/lincomycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI or NotI. The plasmid will integrate into the xkdE gene.


Hints for usage

  • An additional base has to be inserted in the forward primer to restore the frame.
  • Don't forget a stop codon in the reverse primer.


Return to Plasmids