PhrF

• Description: response regulator aspartate phosphatase (RapF) regulator, antagonizes the RapF-ComA interaction
  
  

Gene name	phrF
Synonyms	ywhI
Essential	no
Product	phosphatase (RapF) regulator
Function	control of ComA activity
Gene expression levels in SubtiExpress: phrF
Interactions involving this protein in SubtInteract: PhrF
MW, pI	4 kDa, 10.237
Gene length, protein length	117 bp, 39 aa
Immediate neighbours	rapF, ywhH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

ComA regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU37470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to RapF, this results in the inability of RapF to interact with ComA PubMed

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapF-PhrF PubMed

• Localization:

Database entries

• Structure:

• UniProt: P71001

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rapF-phrF PubMed
  • phrF PubMed

• Expression browser: phrF PubMed

• Sigma factor:
  • phrF: SigH PubMed

• Regulation:
  • PhrF accumulates at high cell density

• Regulatory mechanism:
  • ComA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Melinda D Baker, Matthew B Neiditch
Structural basis of response regulator inhibition by a bacterial anti-activator protein.
PLoS Biol: 2011, 9(12);e1001226
[PubMed:22215984] [WorldCat.org] [DOI] (I p)

Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)