RapJ

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P
  
  

Gene name	rapJ
Synonyms	ycdE
Essential	no
Product	response regulator aspartate phosphatase
Function	control of the phosphorelay
Gene expression levels in SubtiExpress: rapJ
Interactions involving this protein in SubtInteract: RapJ
MW, pI	44 kDa, 4.902
Gene length, protein length	1119 bp, 373 aa
Immediate neighbours	cwlK, ycdF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, phosphorelay, transcription factors and their control

The gene

Basic information

• Locus tag: BSU02820

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • dephosphorylates Spo0F-P PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide PhrC, the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. PubMed
• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • the interaction with PhrC inactivates RapJ PubMed

• Interactions:
  • RapJ-Spo0F, dephosphorylation of Spo0F-P PubMed
  • RapJ-PhrC PubMed

• Localization:

Database entries

• Structure:
  • 4GYO (complex RapJ-PhrC) PubMed

• UniProt: O34327

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapJ (according to DBTBS)

• Expression browser: rapJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: