Difference between revisions of "WalR"

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==Reviews==
 
==Reviews==
 
<pubmed>19019149, 18245295,18792692, 22126136, 10094672 </pubmed>
 
<pubmed>19019149, 18245295,18792692, 22126136, 10094672 </pubmed>
 
 
==The [[WalR regulon]]==
 
==The [[WalR regulon]]==
 
<pubmed>17581128,10878122,12950927, </pubmed>
 
<pubmed>17581128,10878122,12950927, </pubmed>
 
==Other original Publications==
 
==Other original Publications==
'''Additional publications:''' {{PubMed|22122227}}
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<pubmed>18573169,17350627,10094672,17827301,9829949,16030236,19574649, 20059685 20111065 20167622 20834167 24125693 22122227</pubmed>
<pubmed>18573169,17350627,10094672,17827301,9829949,16030236,19574649, 20059685 20111065 20167622 20834167 </pubmed>
 
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:55, 23 October 2013

  • Description: two-component response regulator, controls cell wall metabolism

Gene name walR
Synonyms yycF
Essential yes PubMed
Product two-component response regulator
Function control of cell wall metabolism
Gene expression levels in SubtiExpress: walR
Interactions involving this protein in SubtInteract: WalR
MW, pI 27 kDa, 4.876
Gene length, protein length 705 bp, 235 aa
Immediate neighbours walK, trnY-Phe
Sequences Protein DNA DNA_with_flanks
Genetic context
YycF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
WalR expression.png















Categories containing this gene/protein

cell wall/ other, transcription factors and their control, essential genes, phosphoproteins

This gene is a member of the following regulons

The WalR regulon

The gene

Basic information

  • Locus tag: BSU40410

Phenotypes of a mutant

  • essential PubMed
  • a constitutively active form of WalR (WalR-R204C) results in constitutive expression of the WalR regulon, and in combination with a sepF mutation to the formation of cell wall-less L-forms PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: OmpR family of two-component transcription regulators
  • Paralogous protein(s):


Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated by WalK on an Asp residue
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation likely affects DNA-binding activity

Database entries

  • Structure: 2D1V (DNA binding domain), 2ZWM (receiver domain)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: expressed during vegetative growth, repressed during stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Kevin M Devine
Bacterial L-forms on tap: an improved methodology to generate Bacillus subtilis L-forms heralds a new era of research.
Mol Microbiol: 2012, 83(1);10-3
[PubMed:22126136] [WorldCat.org] [DOI] (I p)

Sarah Dubrac, Paola Bisicchia, Kevin M Devine, Tarek Msadek
A matter of life and death: cell wall homeostasis and the WalKR (YycGF) essential signal transduction pathway.
Mol Microbiol: 2008, 70(6);1307-22
[PubMed:19019149] [WorldCat.org] [DOI] (I p)

Sarah Dubrac, Tarek Msadek
Tearing down the wall: peptidoglycan metabolism and the WalK/WalR (YycG/YycF) essential two-component system.
Adv Exp Med Biol: 2008, 631;214-28
[PubMed:18792692] [WorldCat.org] [DOI] (P p)

Malcolm E Winkler, James A Hoch
Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria.
J Bacteriol: 2008, 190(8);2645-8
[PubMed:18245295] [WorldCat.org] [DOI] (I p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)

The WalR regulon

Other original Publications