Difference between revisions of "PWH844"
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[[File:PWH844_cloning_region.jpeg|250px|thumb|right|'''pWH844 cloning region: click to enlarge''']] | [[File:PWH844_cloning_region.jpeg|250px|thumb|right|'''pWH844 cloning region: click to enlarge''']] | ||
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| − | vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein | + | vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein |
| − | the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | + | the [[plasmid]] carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture |
constructed in the [[Hillen]] lab | constructed in the [[Hillen]] lab | ||
| − | in E. coli: ampicillin resistance | + | in ''E. coli'': ampicillin resistance |
| − | similar plasmid: [[pGP570]] | + | similar [[plasmids|plasmid]]: [[pGP570]] |
sequencing primer: | sequencing primer: | ||
| − | *'''JS39 | + | *<font color="#ff0000">'''JS39'''</font>: 5’-TCTATCAACAGGAGTCCAAGC-3‘ |
*'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | *'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | ||
*'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | *'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | ||
| − | *''' | + | *<font color="#99E500">'''JK37'''</font>: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘ |
| − | + | <pubmed> 9023219 </pubmed> | |
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:23, 8 October 2013
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- JK37: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘
