ScpA

• Description: DNA segregation and condensation protein
  
  

Gene name	scpA
Synonyms	ypuG
Essential	yes PubMed
Product	DNA segregation and condensation protein
Function	maintenance of chromosome structure
Gene expression levels in SubtiExpress: scpA
Interactions involving this protein in SubtInteract: ScpA
MW, pI	29 kDa, 4.788
Gene length, protein length	753 bp, 251 aa
Immediate neighbours	scpB, ypuF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU23220

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: scpA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • present as stable monomer PubMed
  • ScpA-Smc PubMed
  • ScpA-ScpB PubMed

• Localization:
  • nucleoid (Multiple) PubMed
  • the (Smc)2-ScpA-ScpB complex forms two bipolar assemblies on the chromosome, one in each cell half PubMed

Database entries

• Structure:
  • 3ZGX (complex between the head domain of Smc (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of ScpA) PubMed
  • 4I98 (complex between ScpA (aa 1-160)-ScpB (aa 1-183) PubMed

• UniProt: P35154

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: scpA-scpB-ypuI PubMed

• Expression browser: scpA PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)