SpoIVCB

• Description: sporulation-specific sigma factor (SigK) (N-terminal half), other part: spoIIIC

Gene name	spoIVCB
Synonyms	cisB, sigK
Essential	no
Product	RNA polymerase sporulation-specific sigma factor (sigma-K) (N-terminal half)
Function	late mother cell-specific gene expression
Gene expression levels in SubtiExpress: spoIVCB
Interactions involving this protein in SubtInteract: SigK
MW, pI	17 kDa, 8.208
Gene length, protein length	468 bp, 156 aa
Immediate neighbours	nucB, spoIVCA
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

GerE regulon, SigE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU25760

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIVFB-proSigK PubMed
  • SigK-(RpoB-RpoC)

• Localization:

Database entries

• Structure:

• UniProt: P12254

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigK (composed of spoIVCB-spoIIIC) PubMed

• Expression browser: spoIVCB PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed
  • SpoIIID: transcription activation PubMed

• Additional information:
  • SigK is activated by processing of membrane-bound pro-SigK by the SpoIVFB metalloprotease PubMed
  • spoIVCB and spoIIIC form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Arthur Aronson, Purdue University, West Lafayette, USA homepage
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Gu Chen, Xu Zhang
New insights into S2P signaling cascades: regulation, variation, and conservation.
Protein Sci: 2010, 19(11);2015-30
[PubMed:20836086] [WorldCat.org] [DOI] (I p)

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)

The SigK regulon

Takeko Kodama, Takeshi Matsubayashi, Tadayoshi Yanagihara, Hiroyuki Komoto, Katsutoshi Ara, Katsuya Ozaki, Ritsuko Kuwana, Daisuke Imamura, Hiromu Takamatsu, Kazuhito Watabe, Junichi Sekiguchi
A novel small protein of Bacillus subtilis involved in spore germination and spore coat assembly.
Biosci Biotechnol Biochem: 2011, 75(6);1119-28
[PubMed:21670523] [WorldCat.org] [DOI] (I p)

Jessica M Silvaggi, John B Perkins, Richard Losick
Genes for small, noncoding RNAs under sporulation control in Bacillus subtilis.
J Bacteriol: 2006, 188(2);532-41
[PubMed:16385044] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)