Difference between revisions of "YeeK"

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* '''Description:''' spore coat protein <br/><br/>
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* '''Description:''' inner spore coat protein <br/><br/>
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
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|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || protection of the spore
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06850 yeeK]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06850 yeeK]
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[SigK regulon]]}}
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{{SubtiWiki regulon|[[SigK regulon]]}},
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{{SubtiWiki regulon|[[GerE regulon]]}}
  
 
=The gene=
 
=The gene=
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* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 
** inner spore coat  {{PubMed|19933362}},  assembles into the spore integument [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
 
** inner spore coat  {{PubMed|19933362}},  assembles into the spore integument [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
 +
** localization depends on [[CotE]]  {{PubMed|22171814}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yeeK_753265_753702_1 yeeK] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yeeK_753265_753702_1 yeeK] {{PubMed|22383849}}
  
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* '''Sigma factor:''' [[SigK]] {{PubMed|15699190,19060142}}
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* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190,19060142}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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** expression starts 5 hrs after the onset of sporulation [[GerE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
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** expression starts 5 hrs after the onset of [[sporulation]] ([[SigK]], [[GerE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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* '''Mutant:'''
 
* '''Mutant:'''
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** 1S125 ( ''yeeK''::''erm''), {{PubMed|16751597}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S125&Search=1S125 BGSC]
 
 
** 1S125 ( ''yeeK''::''erm''), {{PubMed|16751597}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S125&Search=1S125 BGSC]
 
** 1S125 ( ''yeeK''::''erm''), {{PubMed|16751597}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S125&Search=1S125 BGSC]
  
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=References=
 
=References=
  
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<pubmed>10714992, 19933362,19060142, </pubmed>
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<pubmed>10714992, 19933362,19060142, 22171814</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:04, 13 August 2013

  • Description: inner spore coat protein

Gene name yeeK
Synonyms
Essential no
Product spore coat protein
Function protection of the spore
Gene expression levels in SubtiExpress: yeeK
MW, pI 15 kDa, 6.852
Gene length, protein length 435 bp, 145 aa
Immediate neighbours yeeI, yezE
Sequences Protein DNA DNA_with_flanks
Genetic context
YeeK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YeeK expression.png















Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon, GerE regulon

The gene

Basic information

  • Locus tag: BSU06850

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

CotE, SafA, and SpoVID are required for proper localization, clolocalizes with YabG PubMed

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Hiromu Takamatsu, Daisuke Imamura, Ritsuko Kuwana, Kazuhito Watabe
Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.
J Bacteriol: 2009, 191(4);1220-9
[PubMed:19060142] [WorldCat.org] [DOI] (I p)

H Takamatsu, T Kodama, A Imamura, K Asai, K Kobayashi, T Nakayama, N Ogasawara, K Watabe
The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.
J Bacteriol: 2000, 182(7);1883-8
[PubMed:10714992] [WorldCat.org] [DOI] (P p)

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