Difference between revisions of "YeeK"
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− | * '''Description:''' spore coat protein <br/><br/> | + | * '''Description:''' inner spore coat protein <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein | |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein | ||
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− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || protection of the spore |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06850 yeeK] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06850 yeeK] | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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<br/><br/> | <br/><br/> | ||
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
− | {{SubtiWiki regulon|[[SigK regulon]]}} | + | {{SubtiWiki regulon|[[SigK regulon]]}}, |
+ | {{SubtiWiki regulon|[[GerE regulon]]}} | ||
=The gene= | =The gene= | ||
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* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
** inner spore coat {{PubMed|19933362}}, assembles into the spore integument [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed] | ** inner spore coat {{PubMed|19933362}}, assembles into the spore integument [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed] | ||
+ | ** localization depends on [[CotE]] {{PubMed|22171814}} | ||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yeeK_753265_753702_1 yeeK] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yeeK_753265_753702_1 yeeK] {{PubMed|22383849}} | ||
− | * '''Sigma factor:''' [[SigK]] {{PubMed|15699190,19060142}} | + | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190,19060142}} |
* '''Regulation:''' | * '''Regulation:''' | ||
− | ** expression starts 5 hrs after the onset of sporulation [[GerE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed] | + | ** expression starts 5 hrs after the onset of [[sporulation]] ([[SigK]], [[GerE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
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* '''Mutant:''' | * '''Mutant:''' | ||
− | |||
** 1S125 ( ''yeeK''::''erm''), {{PubMed|16751597}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S125&Search=1S125 BGSC] | ** 1S125 ( ''yeeK''::''erm''), {{PubMed|16751597}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S125&Search=1S125 BGSC] | ||
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=References= | =References= | ||
− | <pubmed>10714992, 19933362,19060142, </pubmed> | + | <pubmed>10714992, 19933362,19060142, 22171814</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 17:04, 13 August 2013
- Description: inner spore coat protein
Gene name | yeeK |
Synonyms | |
Essential | no |
Product | spore coat protein |
Function | protection of the spore |
Gene expression levels in SubtiExpress: yeeK | |
MW, pI | 15 kDa, 6.852 |
Gene length, protein length | 435 bp, 145 aa |
Immediate neighbours | yeeI, yezE |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06850
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31510
- KEGG entry: [2]
- E.C. number:
Additional information
CotE, SafA, and SpoVID are required for proper localization, clolocalizes with YabG PubMed
Expression and regulation
- Operon: yeeK PubMed
- Regulation:
- expression starts 5 hrs after the onset of sporulation (SigK, GerE) PubMed
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814]
[WorldCat.org]
[DOI]
(I p)
Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362]
[WorldCat.org]
[DOI]
(I p)
Hiromu Takamatsu, Daisuke Imamura, Ritsuko Kuwana, Kazuhito Watabe
Expression of yeeK during Bacillus subtilis sporulation and localization of YeeK to the inner spore coat using fluorescence microscopy.
J Bacteriol: 2009, 191(4);1220-9
[PubMed:19060142]
[WorldCat.org]
[DOI]
(I p)
H Takamatsu, T Kodama, A Imamura, K Asai, K Kobayashi, T Nakayama, N Ogasawara, K Watabe
The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.
J Bacteriol: 2000, 182(7);1883-8
[PubMed:10714992]
[WorldCat.org]
[DOI]
(P p)