Difference between revisions of "CdaS"
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* '''Mutant:''' | * '''Mutant:''' | ||
− | ** GP983 (''[[ | + | ** GP983 (''[[cdaS]]''::''ermC''), available in [[Jörg Stülke]]'s lab |
− | ** GP1360 (''[[ | + | ** GP1360 (''[[cdaS]]''::''spc''), available in [[Jörg Stülke]]'s lab |
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 16:42, 13 August 2013
- Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
Gene name | cdaS |
Synonyms | yojJ |
Essential | no |
Product | sporulation-specific diadenylate cyclase |
Function | synthesis of c-di-AMP |
Gene expression levels in SubtiExpress: cdaS | |
MW, pI | 22 kDa, 5.367 |
Gene length, protein length | 621 bp, 207 aa |
Immediate neighbours | yojK, yojI |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU19430
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family:
- Paralogous protein(s): CdaA
Extended information on the protein
- Kinetic information:
- Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)
- UniProt: O31854
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP983 (cdaS::ermC), available in Jörg Stülke's lab
- GP1360 (cdaS::spc), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- His-tag fusion:
- pGP2591 10xHis-cdaS under the control of native promoter (based on pAC7), available in Jörg Stülke's lab**
- pGP2592 10xHis-cdaS-L44F under the control of native promoter (based on pAC7), available in Jörg Stülke's lab**
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications