Difference between revisions of "CdaS"
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
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+ | * '''His-tag fusion:''' | ||
+ | **pGP2591 10xHis-''cdaS'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab** | ||
+ | **pGP2592 10xHis-''cdaS-L44F'' under the control of native promoter (based on [[pAC7]]), available in [[Jörg Stülke]]'s lab** | ||
* '''two-hybrid system:''' | * '''two-hybrid system:''' |
Revision as of 16:41, 13 August 2013
- Description: sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
Gene name | cdaS |
Synonyms | yojJ |
Essential | no |
Product | sporulation-specific diadenylate cyclase |
Function | synthesis of c-di-AMP |
Gene expression levels in SubtiExpress: cdaS | |
MW, pI | 22 kDa, 5.367 |
Gene length, protein length | 621 bp, 207 aa |
Immediate neighbours | yojK, yojI |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU19430
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family:
- Paralogous protein(s): CdaA
Extended information on the protein
- Kinetic information:
- Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 2FB5 (the protein of B. cereus, 48% identity, 81% similarity)
- UniProt: O31854
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP983 (yojJ::ermC), available in Jörg Stülke's lab
- GP1360 (yojJ::spc), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- His-tag fusion:
- pGP2591 10xHis-cdaS under the control of native promoter (based on pAC7), available in Jörg Stülke's lab**
- pGP2592 10xHis-cdaS-L44F under the control of native promoter (based on pAC7), available in Jörg Stülke's lab**
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352]
[WorldCat.org]
[DOI]
(I p)
Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849]
[WorldCat.org]
[DOI]
(I p)
Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650]
[WorldCat.org]
[DOI]
(I p)