Difference between revisions of "BslA"

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(Original publications)
(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
** ''B. subtilis'' W939 ''yuaB''::Cm (ATCC6051 based) {{PubMed|17850253}}
+
** ''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) {{PubMed|17850253}}
** ''B. subtilis'' NRS2097 ''yuaB''::Cm (NCIB3610 based) {{PubMed|18978066}}
+
** ''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) {{PubMed|18978066}}
** ''B. subtilis'' ''yuaB''::Cm (168 based) {{PubMed|21097620}}
+
** ''B. subtilis'' ''bslA''::Cm (168 based) {{PubMed|21097620}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
** Physpank-yuaB based on vector pDR111: pDRyuaB2 {{PubMed|21097620}}
+
** Physpank-bslA based on vector pDR111: pDRyuaB2 {{PubMed|21097620}}
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
** pNW500 P''yuaB-lacZ'' fusion in pDG1663 {{PubMed|18978066}}
+
** pNW500 P''bslA-lacZ'' fusion in pDG1663 {{PubMed|18978066}}
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''
** P''yuaB-gfp'' fusion in pSG1151 vector: pSGyuaB {{PubMed|21097620}}
+
** P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB {{PubMed|21097620}}
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  

Revision as of 07:59, 2 August 2013

  • Description: bacterial hydrophobin, forms water-repellent surface layer of the biofilm, inhibitor of KinA autophosphorylation, and subsequently of entry into sporulation

Gene name bslA
Synonyms yuaB, sivB
Essential no
Product biofilm surface layer, inhibitor of KinA autophosphorylation
Function biofilm formation, control of entry into sporulation via the phosphorelay
Gene expression levels in SubtiExpress: bslA
MW, pI 19 kDa, 9.987
Gene length, protein length 543 bp, 181 aa
Immediate neighbours gbsR, ktrA
Sequences Protein DNA DNA_with_flanks
Genetic context
YuaB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YuaB expression.png















Categories containing this gene/protein

biofilm formation, phosphorelay

This gene is a member of the following regulons

AbrB regulon, DegU regulon

The gene

Basic information

  • Locus tag: BSU31080

Phenotypes of a mutant

  • reduced colony complexity PubMed
  • loss of surface repellency of the biofilm PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): SivA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • extracellular (signal peptide) PubMed
    • biofilm matrix in an extracellular polysaccharide-dependent manner PubMed
    • forms a layer on the biofilm surface PubMed

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expression is down-regulated in a rok mutant (indirect effect) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • B. subtilis W939 bslA::Cm (ATCC6051 based) PubMed
    • B. subtilis NRS2097 bslA::Cm (NCIB3610 based) PubMed
    • B. subtilis bslA::Cm (168 based) PubMed
  • Expression vector:
    • Physpank-bslA based on vector pDR111: pDRyuaB2 PubMed
  • lacZ fusion:
    • pNW500 PbslA-lacZ fusion in pDG1663 PubMed
  • GFP fusion:
    • PbslA-gfp fusion in pSG1151 vector: pSGyuaB PubMed
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications