Difference between revisions of "DgcW"
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* '''Mutant:''' | * '''Mutant:''' | ||
** GP850 (''[[ykoW]]''::''cat''), available in [[Jörg Stülke]]'s lab | ** GP850 (''[[ykoW]]''::''cat''), available in [[Jörg Stülke]]'s lab | ||
+ | ** BP140 (ermC) available in [[Fabian Commichau]]'s lab | ||
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 12:40, 11 July 2013
- Description: potential diguanylate cyclase and phosphodiesterase
Gene name | ykoW |
Synonyms | |
Essential | no |
Product | potential diguanylate cyclase and phosphodiesterase |
Function | unknown |
Gene expression levels in SubtiExpress: ykoW | |
MW, pI | 84 kDa, 6.823 |
Gene length, protein length | 2247 bp, 749 aa |
Immediate neighbours | ykoV, ykoX |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
membrane proteins, metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13420
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- contains a central GGDEF domain and a C-terminal EAL domain PubMed
- aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O34311
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP850 (ykoW::cat), available in Jörg Stülke's lab
- BP140 (ermC) available in Fabian Commichau's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References