Difference between revisions of "PGP172"

Latest revision as of 14:04, 3 June 2013

pGP172: click to enlarge

pGP172 cloning region: click to enlarge

vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

sequencing primer:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

@@ Line 2: / Line 2: @@
 [[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']]
-'''Applications:'''
-Vector pGP172 was designed for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein. The ''E. coli'' strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in ''E. coli''. The plasmid was constructed in the [[Stülke]] lab and it is based on the vector pET3C.  pGP172 is similar to [[plasmid]] [[pGP574]].
+* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
-Sequencing primers:
+* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
-*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
-*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
+* constructed in the [[Stülke]] lab
+* in ''E. coli'': ampicillin resistance
+* based on pET3C
+* similar [[plasmid]]: [[pGP574]]
+* sequencing primer:
+**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
+** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
+* important: ''Eco''RI, although mentioned in the MCS, is not unique!
 <pubmed> 14717704 </pubmed>
+==  Return to [[Plasmids]] ==