Difference between revisions of "PGP172"

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[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']]
 
[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']]
  
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'''Applications:'''
 
  
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Vector pGP172 was designed for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein. The ''E. coli'' strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in ''E. coli''. The plasmid was constructed in the [[Stülke]] lab and it is based on the vector pET3C.  pGP172 is similar to [[plasmid]] [[pGP574]].
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* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
  
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Sequencing primers:  
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* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
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*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
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*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
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* constructed in the [[Stülke]] lab
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* in ''E. coli'': ampicillin resistance
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* based on pET3C
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* similar [[plasmid]]: [[pGP574]]
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* sequencing primer:
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**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
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** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
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* important: ''Eco''RI, although mentioned in the MCS, is not unique!
  
 
<pubmed> 14717704 </pubmed>
 
<pubmed> 14717704 </pubmed>
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==  Return to [[Plasmids]] ==

Latest revision as of 14:04, 3 June 2013

pGP172: click to enlarge
pGP172 cloning region: click to enlarge


  • vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
  • host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  • in E. coli: ampicillin resistance
  • based on pET3C
  • sequencing primer:
    • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
  • important: EcoRI, although mentioned in the MCS, is not unique!

Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)

Return to Plasmids

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