Difference between revisions of "PGP1460"
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| − | The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the [[SPINE]] method. | + | [[File:PGP1460.png|250px|thumb|right|'''pGP1460: click to enlarge''']] |
| + | [[File:PGP382_cloning_region.jpeg|250px|thumb|right|'''pGP382 cloning region: click to enlarge''']] | ||
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| + | The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. The plasmid will integrate into the ''[[ganA]]'' gene. Just like [[pGP382]] it can be used for the [[SPINE]] method. | ||
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| + | The cloning region of [[pGP382]] is shown for detailed information. | ||
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| + | '''Genes cloned into this vector require a Shine-Dalgarno sequence.''' | ||
Sequencing primers: | Sequencing primers: | ||
| − | *''' | + | *'''KG64:''' 5‘-TATCAGGGCCTCGACTACA-3‘ |
| − | *''' | + | *'''ML107:''' 5‘-GCTTCATAGAGTAATTCTGTAAAGG-3‘ |
| + | |||
| + | Similar plasmid: [[pGP1459]] (for N-terminal Strep-tags) | ||
| + | <pubmed> 23192352 </pubmed> | ||
| − | + | == Back to [[Plasmids]] == | |
Latest revision as of 10:11, 28 May 2013
The vector was constructed in the Stülke lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI. The plasmid will integrate into the ganA gene. Just like pGP382 it can be used for the SPINE method.
The cloning region of pGP382 is shown for detailed information.
Genes cloned into this vector require a Shine-Dalgarno sequence.
Sequencing primers:
- KG64: 5‘-TATCAGGGCCTCGACTACA-3‘
- ML107: 5‘-GCTTCATAGAGTAATTCTGTAAAGG-3‘
Similar plasmid: pGP1459 (for N-terminal Strep-tags)
Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352]
[WorldCat.org]
[DOI]
(I p)
