LeuS

• Description: Leu-tRNA synthetase
  
  

Gene name	leuS
Synonyms
Essential	yes PubMed
Product	leucyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: leuS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	91 kDa, 4.857
Gene length, protein length	2412 bp, 804 aa
Immediate neighbours	ytwF, ytvB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU30320

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2AJG (from Escherichia coli, 39% identity, 56% similarity) PubMed

• UniProt: P36430

• KEGG entry: [3]

• E.C. number: 6.1.1.4

Expression and regulation

• Operon: leuS PubMed

• Expression browser: leuS PubMed

• Sigma factor:

• Regulation:
  • induced by leucine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, binding of uncharged tRNA-Leu results in transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

P B Vander Horn, S A Zahler
Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis.
J Bacteriol: 1992, 174(12);3928-35
[PubMed:1317842] [WorldCat.org] [DOI] (P p)