YabJ

• Description: required for activity of PurR
  
  

Gene name	yabJ
Synonyms
Essential	no
Product	unknown
Function	regulation of purine biosynthesis
Gene expression levels in SubtiExpress: yabJ
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	13 kDa, 5.134
Gene length, protein length	375 bp, 125 aa
Immediate neighbours	purR, spoVG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

• Locus tag: BSU00480

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Ref.3 (according to Swiss-Prot) YjgF family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms a trimer PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1QD9, 1QD9 NCBI PubMed

• UniProt: P37552

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: purR-yabJ PubMed

• Expression browser: yabJ PubMed

• Sigma factor:

• Regulation: negative autoregulation PubMed
  • repressed in the presence of purine nucleotides (PurR) PubMed

• Regulatory mechanism: repression by PurR PubMed
  • PurR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

S Sinha, P Rappu, S C Lange, P Mäntsälä, H Zalkin, J L Smith
Crystal structure of Bacillus subtilis YabJ, a purine regulatory protein and member of the highly conserved YjgF family.
Proc Natl Acad Sci U S A: 1999, 96(23);13074-9
[PubMed:10557275] [WorldCat.org] [DOI] (P p)

P Rappu, B S Shin, H Zalkin, P Mäntsälä
A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor.
J Bacteriol: 1999, 181(12);3810-5
[PubMed:10368157] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)