Difference between revisions of "PWH844"
| Line 9: | Line 9: | ||
in E. coli: ampicillin resistance | in E. coli: ampicillin resistance | ||
| − | similar plasmid; pGP570 | + | similar plasmid; [[pGP570]] |
Schirmer et al. (1997) J. Bacteriol. '''179:''' 1329-1336. [http://www.ncbi.nlm.nih.gov/sites/entrez/9023219 PubMed] | Schirmer et al. (1997) J. Bacteriol. '''179:''' 1329-1336. [http://www.ncbi.nlm.nih.gov/sites/entrez/9023219 PubMed] | ||
Revision as of 10:15, 15 January 2009
pWH844
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
similar plasmid; pGP570
Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed
