Difference between revisions of "CimH"

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|style="background:#ABCDEF;" align="center"|'''Function''' || citrate uptake
 
|style="background:#ABCDEF;" align="center"|'''Function''' || citrate uptake
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38770 cimH]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38770 cimH]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cydA]]'', ''[[yxkI]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cydA]]'', ''[[yxkI]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15903&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38770 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yxkJ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yxkJ_context.gif]]

Revision as of 14:12, 13 May 2013

  • Description: transporter for citrate (proton symport)

Gene name cimH
Synonyms yxkJ
Essential no
Product transporter for citrate (proton symport)
Function citrate uptake
Gene expression levels in SubtiExpress: cimH
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 48 kDa, 6.554
Gene length, protein length 1350 bp, 450 aa
Immediate neighbours cydA, yxkI
Sequences Protein DNA Advanced_DNA
Genetic context
YxkJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CimH expression.png



















Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU38770

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: uptake of citrate (symport with protons) PubMed
  • Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family
  • Paralogous protein(s): MaeN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism:
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Bastiaan P Krom, Juke S Lolkema
Conserved residues R420 and Q428 in a cytoplasmic loop of the citrate/malate transporter CimH of Bacillus subtilis are accessible from the external face of the membrane.
Biochemistry: 2003, 42(2);467-74
[PubMed:12525174] [WorldCat.org] [DOI] (P p)

B P Krom, R Aardema, J S Lolkema
Bacillus subtilis YxkJ is a secondary transporter of the 2-hydroxycarboxylate transporter family that transports L-malate and citrate.
J Bacteriol: 2001, 183(20);5862-9
[PubMed:11566984] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)