Difference between revisions of "PGP380"
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| − | Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology | + | Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology |
| + | |||
| + | constructed in [[Stülke]] lab | ||
| + | |||
| + | in ''E. coli'': ampicillin resistance | ||
| + | in B''. subtilis'': erythromycin resistance | ||
| + | |||
| + | based on [[pBQ200]] | ||
| + | |||
| + | similar vector: [[pGP382]] | ||
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17994626 PubMed] | Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17994626 PubMed] | ||
Revision as of 20:49, 14 January 2009
Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology
constructed in Stülke lab
in E. coli: ampicillin resistance in B. subtilis: erythromycin resistance
based on pBQ200
similar vector: pGP382
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed
